Bioequivalence & Bioavailability

The demand to get a tool for biological parameter estimation of new compounds for clinical application, supports the postulation of predictive models as an alternative to conventional classical assays being no necessary the use of experimentation in animals. A lot of in vitro systems, including the use of physicochemical parameters of drugs, the permeability data from cell culture line and the chromatographic models [1-4], have been established. The study of the type of chemical structure of a foreign substance, which will interact to a living system and produce a well-defined biological endpoint, is commonly referred to as quantitative structure-activity relationship (QSAR). The application of chromatographic parameters in QSAR gives rise to a new field: quantitative retention-activity relationship (QRAR) [5-7]. Sagrado has named this methodology BMC [8,9]. Which is a chromatographic modality optimized in order to describe the biological behavior of drugs, comprises a hydrophobic stationary phase and saline solutions of Brij35 micelles as mobile phase, has been testified to be useful for describing and predicting the biological activities of different pharmacological kinds of drugs [10], permeability across the intestinal barrier [11,12], blood–brain barrier and cornea [13].